Bioconversion Study of Olive Tree Biomass Hemicellulosic Hydrolysates by Candida guilliermondii at Different Scales for Ethanol and Xylitol Production

Hemicellulosic biomass from olive-tree pruning (OTPB) was used as a raw material in order to produce hemicellulosic hydrolysate be fermented with the non-traditional yeast Candida guilliermondii FTI 20037 obtain ethanol and xylitol. The main objectives of this research were study most relevant kinetic parameters involved bioconversion process correlation between stirred-tank bioreactor agitated Erlenmeyer flask fermentation. In first scale-up (using flasks) incubated on rotary shaker at 200 rpm, fermentation assays performed determine convenient conditions adaptation microorganism concentrated OTPB added nutrients culture medium. best (2.5 kg m−3 initial cells, pH 5.5 30 °C) set bench bioreactor. A comparative xylitol production conducted two scale scenarios, obtaining different results. bioreactor, 100% D-glucose partially D-xylose consumed an yield 0.28 kg−1 volumetric productivity 0.84 dm−3 h−1 well 0.37 0.26 h−1, respectively. results allowed increasing action more real than previous steps enable mini-plant industrial scaling.